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Procedure : Part 2

Apparatus/ Materials
Cloudy pure apple juice Pectinase enzyme Amylase 1 cm3 syringe 10 cm3 syringe Waterproof marker pen Test tubes Test tube rack Water bath set to 50 ℃	40 ml 1.5 ml 1.5 ml 2 pcs. 1 pc. 1 pc. 4 pcs. 1 pc. 1 pc.
Label test tubes
	The tops of four test tubes are labeled as A, B, C and D respectively by using a marker pen.
Add pectinase
	1 ml of pectinase (P) is added into test tube A. 1 ml of amylase (A) is added into test tube B. Mixture of 0.5 ml of pectinase and 0.5 mlof amylase (P+A) is added into test tube C. Finally, 1 ml of water (W) is added into test tube D.
Add apple juice
	10 ml of apple juice is added into each test tube. The mixture inside the test tubes is stirred gently to distribute any suspended particles evenly.
Mix enzymes
	Stir or agitate the contents of the tubes to mix the enzymes throughout the juice.
50℃ water bath
	Incubate all the test tubes into the water bath at 50℃.
Observe and record
Observe the changes in the tubes and record the appearance of their contents over half an hour at 5 minute intervals
Further Clearing
Result	Further clearing may occur if the tubes are left to stand overnight at room temperature. A = Clear supernatant + cloudy suspension B = Cloudy solution C = Clear solution D = Cloudy solution