Recombinant DNA Technology
Recombinant DNA Technology is based on the strategy devised by Stanley Cone and Herbert Boyar in 1973.
They devised a methodology for transferring genes from one organism to another.
It enables researchers to isolate genes and insert them in host organisms.
It is beneficial to many different areas of study, particularly in biotechnology.
Restriction Enzyme
Gene Cloning
Gene cloning is the reproduction of "foreign" genes in bacteria or other cells.
A clone is a group of cells, organisms, or genes that possesses exact copies of each other.
The "foreign" gene is inserted into the DNA of a culture of bacteria or other cells and forms the recombinant DNA.
Those bacteria or cells are suitable for large production of the desired product.
The recombinant DNA is then replicated as the cells divide and produce many copies of the gene.
Gene Cloning with Different Vectors
Monoclonal Antibodies Production
In 1975, Kohler and Milstein developed hybridoma cells.
Hybridoma cell is found from the fusion of a B cell and cancer cell.
Cancer cells enable the multiplication and growth for a long period.
B cells enable the production of antibodies.
Antibodies produced by hybridoma cell are identical and specific for single antigen.
These antibodies are produced from a single clone of B cells and so call the monoclonal antibodies.
Polymerase Chain Reaction - PCR
In 1985, K.B. Mullis and his colleagues reported the use of polymerase chain reaction (PCR) for enzymatic amplification in vitro.
PCR allows the amplification of specific gene fragments over millionfold.
PCR can be used to obtain a large amount of a specific DNA sequence for cloning.
PCR can also be used in the detection of mutation of DNA in genetic diseases.
PCR can also be used to identify the presence of a pathogenic organism in a biological sample.
Copyright(c)2002 HKIEd. All Rights Reserved.
請採用 Internet Explorer 4.0 或 Netscape Navigator 4.0 或以上瀏覽器，並以800x600解析度瀏覽。
