°ò¦]«²Õ§Þ³N (Recombinant DNA Technology)
Stanley Cohen ©M Herbert Boyer ©ó1973¦~µo©ú¤F°ò¦]«²Õ§Þ³N¡C ³o§Þ³N¬O±N°ò¦]±q¤@¥Íª«ÅéÂಾ¨ì¥t¤@¥Íª«Å餺ªº¡C
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°ò¦]½Æ»s (Gene Cloning)
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µL©ÊÁc´Þ²ÓM¨t(©ÎºÙ§J¶© - Clone)¡A¬O¤@¸s¦³¬Û¦P¿ò¶Ç¯S¼xªºÓÅé²ÓM¸s¡N²Õ´©Î°ò¦]¡C
¥~¨Ó°ò¦]º¥ý©ñ¤J¥t¤@ӥͪ«Å骺DNA¡A»s¦¨«²Õ°ò¦]¡A¨Ãª`¤J²Óµß©Î¨ä¥L²ÓMÅ餺¶i¦æ°ò¦]Ác´Þ¡C
³æ§J¶©§ÜÅé (Monoclonal Antibodies Production)
1975¦~ Kohler ©M Milstein »s³y¤FÂøºØ½F²ÓM®è(Hybridoma cells)¡C
±N¤p¹«ªºB²ÓM (B Cell) »P½F²ÓM (Cancer cell)¿Ä¦X¡A»s¦¨¥i¦bÅé¥~³sÄò°ö¾i¨Ã¯à¤Àªc§ÜÅé (Antibody) ªºÂøºØ½F²ÓM®è¡C
¸Ó²ÓM®è©Ò¤Àªcªº§ÜÅé³£§t¦³¬Û¦Pªº§ÜÅé¤À¤l¸s¡A¥¦Ì³£¹ï³æ¤@ºØ§Üì (Antigen) ªí²{±M¤@©Ê¡C
¥Ñ©ó¦¹§ÜÅé¬O¥ÑB²ÓM³æ§J¶©²£¥Í¡A¬G¦W¬°³æ§J¶©§ÜÅé (Monoclonal antibody)¡C
¦h»EžJÃ즡¤ÏÀ³ (Polymerase Chain Reaction - PCR)
1985¦~¡AK.B. Mullis ©M¥Lªº¦Pµ¡Ìµo©ú¤F¥Î¦h»EžJÃ즡¤ÏÀ³ (PCR) ¤èªk¥h¶i¦æÅé¥~ÂX¼W°ò¦]¡A±N¿ï©wªº°ò¦]¤ù¬q©ñ¤j½Æ»s¹L¦Ê¸U¿¡C
PCRªºÀ³¥Î¬Æ¼s¡A¥¦¥i¥HºÊ©w²Óµßªº¦s¦b¡A¤j¶q½Æ»s¥Í²£©Ò»ÝªºDNA¡A¤Î¶EÂ_¿ò¶Ç¯fµ¥¡C
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